Dragrath
Duke Fishron
Ah this again while there are new things to add nothing really changed from before most bosses aren't really classifiable by gender
King Slime: Slimes are asexual they have no gender
Eye of Cthulhu: ??? Undetermined Eldritch horror attempting to assign the mortal concept of gender here will lead to your sanity level dropping drastically.
Eater of Worlds: Undetermined likely Asexual or Female
Brain of Cthulhu: ??? Undetermined Eldritch horror attempting to assign the mortal concept of gender here will lead to your sanity level dropping drastically.
Queen bee:Like nearly all Bees it would be female male bees only have half a chromosome anyway...
Skeletron: Gender never established but probably once had a gender when they were alive (likely male but once again never established)
Wall of Flesh: ??? Undetermined Eldritch horror attempting to assign the mortal concept of gender here will lead to your sanity level dropping drastically.
Twins: Undetremined Either Asexual if Cyborg or N/A
Destroyer:N/A Machines have no gender seems to be based on EOW and BOC so even if part cyborg it would be undetermined
Skeletron Prime: N/A Machines have no gender no organic parts at any stage of fight
Plantera: Hermaphrodite because it is a plant...
Golem: N/A Machines have no gender even if they are ancient
Duke Fishron: Presumed to be male but never confirmed to have a specific gender.(If they are a species then they would have both sexes names don't matter in nature)
Lunatic Cultist: Considering the Eldric possession going on and fully masked appearance no gender identification can be done
Moon Lord: ??? Undetermined Eldritch horror attempting to assign the mortal concept of gender here will lead to your sanity level dropping drastically.
Flying Dutchmen: Ships named "female" but really it isn't alive so N/A
Martian Saucer: Space ship of Martian origin no further information can be identified it is currently unknown if Martians even have genders nor what ship traditions are used by Martians
Mouring Wood: Undead Plant so Hermaphrodite or undefined(even if it its name is a reference to that part which in humans is male only... It isn't always the case Hyena's for instance but plants are not confined to gender)
Pumpking: Undead spirt plus a plant... basically it is undetermined but assumed to represent a male even if really genderless
Everscream: It is a plant so Hermaphrodite
Santa NK1: N/A Machines have no gender
Ice queen:It is made of ice so really it can't have a gender but usually assumed to be "female"
Dark Mage:Never identified even in original game
Ogre:Never identified even in original game
Betsy:Female one of the few that is obvious to awnser
King Slime: Slimes are asexual they have no gender
Eye of Cthulhu: ??? Undetermined Eldritch horror attempting to assign the mortal concept of gender here will lead to your sanity level dropping drastically.
Eater of Worlds: Undetermined likely Asexual or Female
Brain of Cthulhu: ??? Undetermined Eldritch horror attempting to assign the mortal concept of gender here will lead to your sanity level dropping drastically.
Queen bee:Like nearly all Bees it would be female male bees only have half a chromosome anyway...
Skeletron: Gender never established but probably once had a gender when they were alive (likely male but once again never established)
Wall of Flesh: ??? Undetermined Eldritch horror attempting to assign the mortal concept of gender here will lead to your sanity level dropping drastically.
Twins: Undetremined Either Asexual if Cyborg or N/A
Destroyer:N/A Machines have no gender seems to be based on EOW and BOC so even if part cyborg it would be undetermined
Skeletron Prime: N/A Machines have no gender no organic parts at any stage of fight
Plantera: Hermaphrodite because it is a plant...
Golem: N/A Machines have no gender even if they are ancient
Duke Fishron: Presumed to be male but never confirmed to have a specific gender.(If they are a species then they would have both sexes names don't matter in nature)
Lunatic Cultist: Considering the Eldric possession going on and fully masked appearance no gender identification can be done
Moon Lord: ??? Undetermined Eldritch horror attempting to assign the mortal concept of gender here will lead to your sanity level dropping drastically.
Flying Dutchmen: Ships named "female" but really it isn't alive so N/A
Martian Saucer: Space ship of Martian origin no further information can be identified it is currently unknown if Martians even have genders nor what ship traditions are used by Martians
Mouring Wood: Undead Plant so Hermaphrodite or undefined(even if it its name is a reference to that part which in humans is male only... It isn't always the case Hyena's for instance but plants are not confined to gender)
Pumpking: Undead spirt plus a plant... basically it is undetermined but assumed to represent a male even if really genderless
Everscream: It is a plant so Hermaphrodite
Santa NK1: N/A Machines have no gender
Ice queen:It is made of ice so really it can't have a gender but usually assumed to be "female"
Dark Mage:Never identified even in original game
Ogre:Never identified even in original game
Betsy:Female one of the few that is obvious to awnser